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KMID : 0545120140240091254
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 9 p.1254 ~ p.1259
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Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of β-Galactosidase-Containing Escherichia coli
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Lee Hyang-Yeol
Jung Kyung-Hwan
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Abstract
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We investigated whether β-galactosidase (β-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the β-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7―8 times greater than that of a previous report. In addition, the concentration of β-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.
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KEYWORD
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2-phenoxyethanol, β-galactosidase, transgalactosylation, 2-phenoxyethanol galactoside, Escherichia coli
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